Activated Resins – Biotoolomics’ Many Options
BioToolomics has developed an extensive range of activated media with a wide range of coupling chemistries for efficient, fast, easy, and safe immobilization of various functional ligands. The agarose base matrix is very hydrophilic and exhibits very low nonspecific adsorption. In addition, it is highly cross-linked for mechanical rigidity, allowing high flow rates with reduced back pressure. The pore structures have been carefully designed for the coupling of small or large ligands. High selectivity and high capacity allow flexible custom immobilization techniques ideally suited to the isolation of specific molecules from complex biological feedstocks.
Biotoolomics offers a wide range of Activated Resin options, including:
Aldehyde-activated SepFast Resins and Aldehyde Activated Magnetic Resins - The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents to form chemically stable secondary amine linkages.
Amine-activated SepFast and Amine-activated Magnetic Resins - The base matrix is activated by attaching a long hydrophilic chain with an amine group at the end. This reacts with either carboxyl containing ligands in the presence of carbodiimide agent, or aldehyde containing ligands in the presence of reducing agent. The coupling chemistry is a successful and well-documented technique.
Carboxyl-activated SepFast and Carboxyl-activated Magnetic Resins - The base matrix is activated by attaching a long 10 atom hydrophilic chain with a carboxyl group at the end. This reacts with amine containing ligands in the presence of a carbodiimide agent to form a chemically stable amide linkage. The coupling chemistry is well documented.
CNBr-activated SepFast and CNBr-activated Magnetic Resins - The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized. CNBr-activated agarose beads have a proven track record for preparation and use of custom affinity chromatography media.
Epoxy-activated SepFast and Epoxy-activated Magnetic Resins - Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemicals or special equipment is required. All the chemical bonds formed are very stable.
Hydrazide-activated SepFast and Hydrazide-activated Magnetic Resins - The base matrix is activated with hydrazide through a long hydrophilic spacer arm. Hydrazide-activated agarose beads permit the coupling of aldehyde or ketone containing ligands through the formation of stable hydrazone linkages. It is a particularly powerful way to immobilize glycoproteins via their carbohydrate chains while leaving their critical active sites intact. It is a method known as “site-directed immobilization”.
Irreversible Thiol-coupling SepFast and Irreversible Thiol-coupling Magnetic Resins - The base matrix is activated by attaching a haloacetyl group using a long hydrophilic spacer arm which facilitates much improved ligand accessibility. It reacts rapidly with molecules containing thiol groups.
NHS-activated SepFast and NHS-activated Magnetic Resins - The base matrix is activated using a long hydrophilic spacer arm with N-hydroxysuccinimide at the end. This facilitates the immobilization of structurally restricted ligands such as proteins and peptides. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds.